plenti cmv blast Search Results


pip fucci 2 color fluorescent cell cycle reporter plasmid  (Addgene inc)
93
Addgene inc pip fucci 2 color fluorescent cell cycle reporter plasmid
Pip Fucci 2 Color Fluorescent Cell Cycle Reporter Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pip fucci 2 color fluorescent cell cycle reporter plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
pip fucci 2 color fluorescent cell cycle reporter plasmid - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
plenti cmv v5 luc blast  (Addgene inc)
93
Addgene inc plenti cmv v5 luc blast
Plenti Cmv V5 Luc Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti cmv v5 luc blast/product/Addgene inc
Average 93 stars, based on 1 article reviews
plenti cmv v5 luc blast - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
plenti cmv rtta3 blast  (Addgene inc)
94
Addgene inc plenti cmv rtta3 blast
Plenti Cmv Rtta3 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti cmv rtta3 blast/product/Addgene inc
Average 94 stars, based on 1 article reviews
plenti cmv rtta3 blast - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
plenti cmv tre3g mct1  (Addgene inc)
92
Addgene inc plenti cmv tre3g mct1
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Plenti Cmv Tre3g Mct1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti cmv tre3g mct1/product/Addgene inc
Average 92 stars, based on 1 article reviews
plenti cmv tre3g mct1 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
plenticmvblastdest  (Addgene inc)
94
Addgene inc plenticmvblastdest
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Plenticmvblastdest, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenticmvblastdest/product/Addgene inc
Average 94 stars, based on 1 article reviews
plenticmvblastdest - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
plenti cmv blast empty w263 1  (Addgene inc)
94
Addgene inc plenti cmv blast empty w263 1
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Plenti Cmv Blast Empty W263 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti cmv blast empty w263 1/product/Addgene inc
Average 94 stars, based on 1 article reviews
plenti cmv blast empty w263 1 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
cmv tetr blast  (Addgene inc)
93
Addgene inc cmv tetr blast
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Cmv Tetr Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cmv tetr blast/product/Addgene inc
Average 93 stars, based on 1 article reviews
cmv tetr blast - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
gfp rab5  (Addgene inc)
94
Addgene inc gfp rab5
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Gfp Rab5, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gfp rab5/product/Addgene inc
Average 94 stars, based on 1 article reviews
gfp rab5 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
cmv blast dntgfbr2 ha  (Addgene inc)
93
Addgene inc cmv blast dntgfbr2 ha
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Cmv Blast Dntgfbr2 Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cmv blast dntgfbr2 ha/product/Addgene inc
Average 93 stars, based on 1 article reviews
cmv blast dntgfbr2 ha - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
paper addgene plasmid  (Addgene inc)
90
Addgene inc paper addgene plasmid
(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of <t>MCT1</t> and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.
Paper Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/paper addgene plasmid/product/Addgene inc
Average 90 stars, based on 1 article reviews
paper addgene plasmid - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
plenti-cmv-blast-nlrc3 (ovnlrc3) vectors  (Broad Institute Inc)
90
Broad Institute Inc plenti-cmv-blast-nlrc3 (ovnlrc3) vectors
Overexpression of NLRC3 inhibits NALP3-induced IL-1β secretion. A and B, IL-1β secretion in ectopically assembled NALP3 inflammasomes in HEK293FT cells transfected with 500 ng of pro-IL-1β, 250 ng of ASC, and 250 ng of pro-caspase-1 plasmids and the indicated amount of NALP3 (A) or 1 μg of NALP3 and the indicated amount of NLRC3 (B). Each condition was duplicated. Secreted IL-1β was quantified 24 h after transfection in cell-free supernatants by ELISA. C, IL-1β cleavage in HEK293FT cells with ectopically assembled NALP3 inflammasomes. NT, non-transfected. D, NLRC3 mRNA levels in the THP-1 OvCont and <t>OvNLRC3</t> cell lines. Quantitative PCR results of relative mRNA of NLRC3/β-actin are given. The NLRC3/β-actin ratio of OvCont cells was assigned as 1 arbitrary unit. E, NLRC3 protein levels in THP-1 OvControl and OvNLRC3 stable lines. F and G, IL-1β secretion in THP-1 OvNLRC3 and THP-1 OvControl stable lines in response to nigericin (F) and MSU (G) treatment.
Plenti Cmv Blast Nlrc3 (Ovnlrc3) Vectors, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti-cmv-blast-nlrc3 (ovnlrc3) vectors/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
plenti-cmv-blast-nlrc3 (ovnlrc3) vectors - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


(A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of MCT1 and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Activity of the Monocarboxylate Transporter 1 inhibitor AZD3965 in Small Cell Lung Cancer

doi: 10.1158/1078-0432.CCR-13-2270

Figure Lengend Snippet: (A) Normoxic or hypoxic COR-L103, NCI-H1048, DMS79 or DMS114 cells were treated with 8 nM AZD3965 for 72 hours. Expression of MCT1 and MCT4 protein was assayed by western blotting. Tubulin was used as a loading control. (B-D) NCI-H1048 cells engineered to overexpress GFP or MCT4 upon the addition of doxycycline (Dox) were cultured under normoxia or hypoxia and treated with 1 μg/ml doxycycline alone (B) or with the indicated concentration of AZD3965 (C) or 8 nM AZD3965 (D). MCT4 and CA IX expression was assayed by western blotting with tubulin used as loading control (B). Cellular biomass was determined as in Figure 1B (C). Intracellular lactate was measured as in Figure 1D and expressed relative to untreated normoxic or hypoxic cells (D). Blots are representative of three independent experiments. All graphs represent mean of at least three independent experiments ± SEM. * p<0.05 according to paired two tailed t-test.

Article Snippet: Viruses were generated by co-transfecting Lenti-X™ 293T cells with pLenti-CMV-TRE3G-MCT1, MCT4 or GFP or pLenti-CMV-rtTA3G-Blast (Addgene #31797) with pCMV-R8.91 and pMD2.G (kind gift from Dr. Akira Orimo) according to manufacturer’s instructions.

Techniques: Expressing, Western Blot, Cell Culture, Concentration Assay, Two Tailed Test

A SCLC TMA was stained with anti-MCT1, MCT4 and CA IX antibodies and independently scored by two operators. (A) Images of serial sections stained with MCT1, MCT4 and CA IX from two representative cores. Scale bar represents 100 μm. (B) Dot plot of MCT1 vs. MCT4 score (extent × intensity) for each case with a CA IX score >0 (n=70). Dotted lines represent median MCT1 and MCT4 score from all cases. (C) and (D) Kaplan-Meier plots and log-rank p-values for patient survival stratified by low (<median) and high (≥median) expression of MCT1 (C) and MCT4 (D) (n=47).

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Activity of the Monocarboxylate Transporter 1 inhibitor AZD3965 in Small Cell Lung Cancer

doi: 10.1158/1078-0432.CCR-13-2270

Figure Lengend Snippet: A SCLC TMA was stained with anti-MCT1, MCT4 and CA IX antibodies and independently scored by two operators. (A) Images of serial sections stained with MCT1, MCT4 and CA IX from two representative cores. Scale bar represents 100 μm. (B) Dot plot of MCT1 vs. MCT4 score (extent × intensity) for each case with a CA IX score >0 (n=70). Dotted lines represent median MCT1 and MCT4 score from all cases. (C) and (D) Kaplan-Meier plots and log-rank p-values for patient survival stratified by low (

Article Snippet: Viruses were generated by co-transfecting Lenti-X™ 293T cells with pLenti-CMV-TRE3G-MCT1, MCT4 or GFP or pLenti-CMV-rtTA3G-Blast (Addgene #31797) with pCMV-R8.91 and pMD2.G (kind gift from Dr. Akira Orimo) according to manufacturer’s instructions.

Techniques: Staining, Expressing

Overexpression of NLRC3 inhibits NALP3-induced IL-1β secretion. A and B, IL-1β secretion in ectopically assembled NALP3 inflammasomes in HEK293FT cells transfected with 500 ng of pro-IL-1β, 250 ng of ASC, and 250 ng of pro-caspase-1 plasmids and the indicated amount of NALP3 (A) or 1 μg of NALP3 and the indicated amount of NLRC3 (B). Each condition was duplicated. Secreted IL-1β was quantified 24 h after transfection in cell-free supernatants by ELISA. C, IL-1β cleavage in HEK293FT cells with ectopically assembled NALP3 inflammasomes. NT, non-transfected. D, NLRC3 mRNA levels in the THP-1 OvCont and OvNLRC3 cell lines. Quantitative PCR results of relative mRNA of NLRC3/β-actin are given. The NLRC3/β-actin ratio of OvCont cells was assigned as 1 arbitrary unit. E, NLRC3 protein levels in THP-1 OvControl and OvNLRC3 stable lines. F and G, IL-1β secretion in THP-1 OvNLRC3 and THP-1 OvControl stable lines in response to nigericin (F) and MSU (G) treatment.

Journal: The Journal of Biological Chemistry

Article Title: NLRC3 protein inhibits inflammation by disrupting NALP3 inflammasome assembly via competition with the adaptor protein ASC for pro-caspase-1 binding

doi: 10.1074/jbc.M116.769695

Figure Lengend Snippet: Overexpression of NLRC3 inhibits NALP3-induced IL-1β secretion. A and B, IL-1β secretion in ectopically assembled NALP3 inflammasomes in HEK293FT cells transfected with 500 ng of pro-IL-1β, 250 ng of ASC, and 250 ng of pro-caspase-1 plasmids and the indicated amount of NALP3 (A) or 1 μg of NALP3 and the indicated amount of NLRC3 (B). Each condition was duplicated. Secreted IL-1β was quantified 24 h after transfection in cell-free supernatants by ELISA. C, IL-1β cleavage in HEK293FT cells with ectopically assembled NALP3 inflammasomes. NT, non-transfected. D, NLRC3 mRNA levels in the THP-1 OvCont and OvNLRC3 cell lines. Quantitative PCR results of relative mRNA of NLRC3/β-actin are given. The NLRC3/β-actin ratio of OvCont cells was assigned as 1 arbitrary unit. E, NLRC3 protein levels in THP-1 OvControl and OvNLRC3 stable lines. F and G, IL-1β secretion in THP-1 OvNLRC3 and THP-1 OvControl stable lines in response to nigericin (F) and MSU (G) treatment.

Article Snippet: Prof. Ömer Yilmaz, Broad Institute, Massachusetts Institute of Technology, Boston, MA) or newly generated pLenti-CMV-Blast-NLRC3 (OvNLRC3) vectors and their control pLenti-CMV-Blast (named OvCont) together with the helper plasmids pMD2G and psPAX2.

Techniques: Over Expression, Transfection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction